D-Lactate dehydrogenase of Peptostreptococcus elsdenii

Presence of lactate dehydrogenase and lactate racemase in Megasphaera elsdenii grown on glucose or lactate.

Activity of D-lactate dehydrogenase (D-LDH) was shown not only in cell extracts from Megasphaera elsdenii grown on DL-lactate, but also in cell extracts from glucose-grown cells, although glucose-grown cells contained approximately half as much D-LDH as DL-lactate-grown cells. This indicates that the D-LDH of M. elsdenii is a constitutive enzyme. However, lactate racemase (LR) activity was pres...

Peptostreptococcus elsdenii from the caecum of pigs.

Large, Gram-negative, anaerobic cocci (LC) fermenting lactate were first isolated by enrichment culture from the sheep rumen (Elsden, Volcani, Gilchrist & Lewis, 1956). High counts (about 109/ml.) were obtained from young calves but not from adult ruminants by Hobson, Mann & Oxford (1958). The cocci were cultured in numbers of 1 0 6 to 103/ml. from the rumen of cattle fed high-grain rations and...

Electron-nuclear double resonance study of flavodoxin from Peptostreptococcus elsdenii.

Purification and properties of electron-transferring flavoprotein from Peptostreptococcus elsdenii.

An electron-transferring flavoprotein (ETF) which couples the oxidation of NADH to the reduction of butyryl coenzyme A dehydrogenase has been purified in large quantities from the anaerobic bacterium Peptostreptococcus elsdenii. The purified protein appears to be homogeneous on the basis of polyacrylamide disc gel electrophoresis, thin layer isoelectric focusing, sedimentation velocity, and the...

Identification and structure of a novel flavin prosthetic group associated with reduced nicotinamide adenine dinucleotide dehydrogenase from Peptostreptococcus elsdenii.

A novel prosthetic group, which is present in NADH dehydrogenase (EC 1.6.99.3) from Pepfosfrejfococcus etsdenii, has been purified and its physicochemical properties investigated. The chemical structure of this species has been established as the FAD analogue of 7-methyl-B-hydroxyisoalloxazine. The proposed structure is confirmed by: (a) chemical synthesis of 7-methyl-S-hydroxy-isoahoxazine mod...